in fusion hd cloning kit (TaKaRa)
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in fusion hd cloning kit
In Fusion Hd Cloning Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 10081 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/in+fusion+hd+cloning+kit/In-Fusion+HD+Cloning+Kit/pmc13123328-130-12-16
Average 99 stars, based on 10081 article reviews
In Fusion Hd Cloning Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 10081 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/in+fusion+hd+cloning+kit/In-Fusion+HD+Cloning+Kit/pmc13123328-130-12-16
Average 99 stars, based on 10081 article reviews
in fusion hd cloning kit - by Bioz Stars,
2026-09
99/100 stars
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Polymerase Chain Reaction:Article Title: A transferable SARS-CoV-2 IRES module enables dual translation initiation for enhanced antigen expression in COVID-19 mRNA vaccines Article Snippet: Total RNA was extracted from HEK293T cells using TRIzol reagent (Invitrogen, Waltham, MA, USA; cat. no. 15596018), and reverse transcription targeting the 4E-BP1 gene (NCBI GeneBank: NM_004095.4 ) was performed using ImProm-II Reverse Transcriptase (Promega, Madison, WI, USA; cat. no. A3803) and a reverse primer 5′-TTA AAT GTC CAT CTC AAA CTG TGA CTC TTC-3′. .. The resulting cDNA encoding 4E-BP1 was PCR-amplified and cloned into the pcDNA3.1 vector (Invitrogen; cat. no. V79020) using the Clone Assay:Article Title: A transferable SARS-CoV-2 IRES module enables dual translation initiation for enhanced antigen expression in COVID-19 mRNA vaccines Article Snippet: Total RNA was extracted from HEK293T cells using TRIzol reagent (Invitrogen, Waltham, MA, USA; cat. no. 15596018), and reverse transcription targeting the 4E-BP1 gene (NCBI GeneBank: NM_004095.4 ) was performed using ImProm-II Reverse Transcriptase (Promega, Madison, WI, USA; cat. no. A3803) and a reverse primer 5′-TTA AAT GTC CAT CTC AAA CTG TGA CTC TTC-3′. .. The resulting cDNA encoding 4E-BP1 was PCR-amplified and cloned into the pcDNA3.1 vector (Invitrogen; cat. no. V79020) using the Plasmid Preparation:Article Title: A transferable SARS-CoV-2 IRES module enables dual translation initiation for enhanced antigen expression in COVID-19 mRNA vaccines Article Snippet: Total RNA was extracted from HEK293T cells using TRIzol reagent (Invitrogen, Waltham, MA, USA; cat. no. 15596018), and reverse transcription targeting the 4E-BP1 gene (NCBI GeneBank: NM_004095.4 ) was performed using ImProm-II Reverse Transcriptase (Promega, Madison, WI, USA; cat. no. A3803) and a reverse primer 5′-TTA AAT GTC CAT CTC AAA CTG TGA CTC TTC-3′. .. The resulting cDNA encoding 4E-BP1 was PCR-amplified and cloned into the pcDNA3.1 vector (Invitrogen; cat. no. V79020) using the Article Title: Gene encoding cytochrome P450 and use thereof Article Snippet: .. The obtained DNA fragment and pMLH7133 vector, having been digested with the restriction enzymes XbaI/SacI, were linked to each other with Cloning:Article Title: A transferable SARS-CoV-2 IRES module enables dual translation initiation for enhanced antigen expression in COVID-19 mRNA vaccines Article Snippet: Total RNA was extracted from HEK293T cells using TRIzol reagent (Invitrogen, Waltham, MA, USA; cat. no. 15596018), and reverse transcription targeting the 4E-BP1 gene (NCBI GeneBank: NM_004095.4 ) was performed using ImProm-II Reverse Transcriptase (Promega, Madison, WI, USA; cat. no. A3803) and a reverse primer 5′-TTA AAT GTC CAT CTC AAA CTG TGA CTC TTC-3′. .. The resulting cDNA encoding 4E-BP1 was PCR-amplified and cloned into the pcDNA3.1 vector (Invitrogen; cat. no. V79020) using the Article Title: A transferable SARS-CoV-2 IRES module enables dual translation initiation for enhanced antigen expression in COVID-19 mRNA vaccines Article Snippet: .. Various SCoV2 genome-derived cis -acting sequences were inserted in place of the HCV IRES sequence using the Article Title: Biosynthesis of patchoulol via metabolic engineering the oleaginous red yeast Rhodotorula toruloides Article Snippet: All genes used in this study were synthesized by Synbio Technologies (Suzhou, China) or cloned directly from the R. toruloides genomic DNA. .. The DNA Ligation Kit Ver.2.1 (Takara) and Article Title: A transferable SARS-CoV-2 IRES module enables dual translation initiation for enhanced antigen expression in COVID-19 mRNA vaccines Article Snippet: Single residue changes were introduced into plasmids using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, Santa Clara, CA, USA; cat. no. 200521). .. Plasmids with multiple residue changes or deletions were generated using the Article Title: A novel 2-piperazino-pyrimidine compound exhibits asexual antimalarial activity by targeting Plasmodium falciparum plasmepsin X Article Snippet: .. Amplified donor DNA fragments were inserted into the Nco I site using Article Title: Evidence of transfer of antimicrobial resistance genes from the porcine pathogen Streptococcus suis to human clinical isolates of Streptococcus agalactiae in a major pig-producing region of Spain Article Snippet: .. The three amplicons were purified with the FavorPrepTM GEL/PCR Purification Kit (Favorgen, Taiwan) and fused using the Article Title: Gene encoding cytochrome P450 and use thereof Article Snippet: .. The obtained DNA fragment and pMLH7133 vector, having been digested with the restriction enzymes XbaI/SacI, were linked to each other with Sequencing:Article Title: A transferable SARS-CoV-2 IRES module enables dual translation initiation for enhanced antigen expression in COVID-19 mRNA vaccines Article Snippet: .. Various SCoV2 genome-derived cis -acting sequences were inserted in place of the HCV IRES sequence using the Activity Assay:Article Title: A transferable SARS-CoV-2 IRES module enables dual translation initiation for enhanced antigen expression in COVID-19 mRNA vaccines Article Snippet: .. Various SCoV2 genome-derived cis -acting sequences were inserted in place of the HCV IRES sequence using the DNA Ligation:Article Title: Biosynthesis of patchoulol via metabolic engineering the oleaginous red yeast Rhodotorula toruloides Article Snippet: All genes used in this study were synthesized by Synbio Technologies (Suzhou, China) or cloned directly from the R. toruloides genomic DNA. .. The DNA Ligation Kit Ver.2.1 (Takara) and Residue:Article Title: A transferable SARS-CoV-2 IRES module enables dual translation initiation for enhanced antigen expression in COVID-19 mRNA vaccines Article Snippet: Single residue changes were introduced into plasmids using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, Santa Clara, CA, USA; cat. no. 200521). .. Plasmids with multiple residue changes or deletions were generated using the Generated:Article Title: A transferable SARS-CoV-2 IRES module enables dual translation initiation for enhanced antigen expression in COVID-19 mRNA vaccines Article Snippet: Single residue changes were introduced into plasmids using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, Santa Clara, CA, USA; cat. no. 200521). .. Plasmids with multiple residue changes or deletions were generated using the Amplification:Article Title: A novel 2-piperazino-pyrimidine compound exhibits asexual antimalarial activity by targeting Plasmodium falciparum plasmepsin X Article Snippet: .. Amplified donor DNA fragments were inserted into the Nco I site using Purification:Article Title: Evidence of transfer of antimicrobial resistance genes from the porcine pathogen Streptococcus suis to human clinical isolates of Streptococcus agalactiae in a major pig-producing region of Spain Article Snippet: .. The three amplicons were purified with the FavorPrepTM GEL/PCR Purification Kit (Favorgen, Taiwan) and fused using the |